China successfully developed animal husbandry full-chain DNA traceability system based on RFID and other technologies

Recently, it was learned that the Laboratory of Quality and Safety of Animal Products of the Institute of Agricultural Quality Standards and Testing Technology of the Chinese Academy of Agricultural Sciences carried out the research on traceability identification technology of pig and beef products based on DNA molecular marker technology. It was established in China for the first time throughout the "animal breeding - slaughtering". - Processing - Sales" DNA traceability system for the entire chain. DNA as a genetic material has individual uniqueness, processing stability and unchangeability. Each cow, pig, sheep individual, etc. has its own specific DNA fingerprint, and this DNA fingerprint is taken from the livestock into the slaughter, processing and becoming a living. The cooked meat products are stable, unchangeable and detectable. The DNA traceability system established by this feature can truly record the livestock production flow and the quality and safety information of animal products in China, and combine the information traceability system to ensure the accuracy and reliability of the data.

目前,中国的动物产品搜索系统based on RFID (radio frequency identification), bar code QR code and other Internet and Internet of Things technologies has improved the level of animal husbandry production management and animal product quality and safety. However, the limitations of the information traceability system are also very obvious. In response to this issue, under the auspices of the public welfare agricultural industry science and technology project and the Beijing Municipal Science and Technology Commission food safety project, the Institute of Agricultural Quality Standards and Testing Technology of the Chinese Academy of Agricultural Sciences launched this Research, so as to improve the government's relevant departments on animal husbandry disease prevention and control and animal product quality and safety supervision.

The principle of this animal husbandry DNA traceability system is that when animal animals are just in the bar or when they are ear tagged, their blood samples are collected for storage, and the encoded information such as traditional RFID or QR code is recorded. When the animals are slaughtered, sold, transported and transported, and finally enter the supermarket table, the meat samples to be tested are collected. Then, the original preserved biological sample is found through traditional information traceability, and the DNA information analysis is performed on the two samples at the same time. If the DNA fingerprint is the same, the same individual is proved, and the traceability information is proved to be reliable and accurate, and vice versa, the traceability information is not true.

Yang Yuming, chief of the source and process control technology innovation team of animal source products, said that one of the key technologies of DNA traceability system is to design and develop an ear tag blood spot card, which is specially modified after embedding a traditional ear tag. The filter paper is automatically collected on the filter paper while the ear tag is given to the animal, and can be cut and stored together with the ear tag for slaughter. The advantage of this technology is that it does not require additional special collection of biological samples, and does not increase the workload of the enterprise; at the same time, the filter paper can store DNA at room temperature for more than one year, without the need of a low-temperature refrigerator, ordinary file cabinets can be saved; each filter paper costs less than 0.2 yuan, also Will not increase the financial burden of the enterprise too much.

Another key technology for livestock DNA traceability systems is the development of a simple and reliable microsatellite DNA fingerprinting technique. Through the screening of microsatellite markers such as pigs and cattle that have been reported at home and abroad, it was determined that 16 microsatellite markers can be used for the individual traceability of DNA in Chinese beef cattle, the error rate is less than 1/1010; the same can be used for pigs in China. The trace rate of 23 microsatellite markers is less than 1/1016. In 2015, the number of beef cattle slaughtered in China was 50 million, which means that the use of this technology can realize the individual traceability identification of all cattle in China.